The medium is an optimized, chemically defined formulation designed to support the high-density culture and transient transfection of HEK293 cell line in suspension, such as HEK293F, HEK293H, HEK293S and HEK293FT.
Product Characteristics
1. Contains L-glutamine, no need to add separately when using.
2. The medium is chemically defined formulation. This chemically defined medium does not contain any protein, undefined lysates or components of animal origin and has low lot-to-lot variation.
3. No need to change medium before and after transfection, suitable for transient transfection, stable transfection, and cell culture.
4. Suitable for cell suspension culture and large-scale reactor culture, thousands of successful cases of protein expression.
Technical Parameters
Catalog Number | UP1000 | specification | 1000 mL |
Appearance | clear red solution | Applicable cell line | HEK293 related cells（such as HEK293F、HEK293H 、HEK293S、HEK293FT） |
pH | 6.9–7.4 | Generation time | < 26 hrs （The numerical value varied with the cell type and state） |
Sterility test | Asepsis | Evaluation of cell culture | excellent |
Endotoxin | < 10 EU/mL | Product Usage | For scientific research only, not suitable for human or animal diagnosis and treatment. |
Period of validity | 12 months | Storage conditions | 2-8 ℃, keep away from light |
※Note: Union 293 cell culture medium is a clear red transparent liquid. It is kept in the dark at 2 ~ 8 ° C. Do not use the medium if it becomes cloudy or precipitated.
Product use effect:
Union 293 medium is especially suitable for HEK293f culture. The figure below shows the comparison of the culture effect of UNION culture medium and a foreign brand culture medium on Expi293 cells from two aspects of cell growth curve and cell viability.
Note: solid represents cell viability, while hollow represents cell number.
List of some customers:
Fudan University
National protein facility Center
Southern Medical University
Shanghai Jiaotong University
Shanghai University of science and technology
University of science and technology of China
Chinese Academy of Medical Sciences
Shanghai Institute of life sciences, Chinese Academy of Sciences
...
