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293 Cell Culture Medium

The medium is an optimized, chemically defined formulation designed to support the high-density culture and transient transfection of HEK293 cell line in suspension, such as HEK293F, HEK293H, HEK293S and HEK293FT.


Product Characteristics

1.  Contains L-glutamine, no need to add separately when using.

2.  The medium is chemically defined formulation. This chemically defined medium does not contain any protein, undefined lysates or components of animal origin and has low lot-to-lot variation.

3.   No need to change medium before and after transfection, suitable for transient transfection, stable transfection, and cell culture.

4.   Suitable for cell suspension culture and large-scale reactor culture, thousands of successful cases of protein expression.


Technical Parameters





1000 mL


clear red solution

Applicable cell line

HEK293 related cellssuch as HEK293FHEK293H HEK293SHEK293FT



Generation time

< 26 hrs

   The numerical   value varied with the cell type and state

Sterility test


Evaluation of cell culture



< 10 EU/mL

Product Usage

For scientific research only, not suitable for   human or animal diagnosis and treatment.

Period of validity

12 months

Storage conditions

2-8 ℃, keep away from light

※Note: Union 293 cell culture medium is a clear red transparent liquid. It is kept in the dark at 2 ~ 8 ° C. Do not use the medium if it becomes cloudy or precipitated.

Product use effect:

Union 293 medium is especially suitable for HEK293f culture. The figure below shows the comparison of the culture effect of UNION culture medium and a foreign brand culture medium on Expi293 cells from two aspects of cell growth curve and cell viability.

Note: solid represents cell viability, while hollow represents cell number.

List of some customers:

Fudan University

National protein facility Center

Southern Medical University

Shanghai Jiaotong University

Shanghai University of science and technology

University of science and technology of China

Chinese Academy of Medical Sciences

Shanghai Institute of life sciences, Chinese Academy of Sciences


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