Product Description
1. The operating system is flexible and simple, allowing users to customize the configuration of hardware and software to meet a variety of purification needs.
2. Dual plunger and binary gradient infusion pump and imported PEEK pump head to ensure high precision and reproducibility during the separation and purification process.
3. Imported DAD full-spectrum detector is used for the UV detection, which has high wavelength precision and good repeatability. Dual channel simultaneous detection is to improve the convenience of detection.
4. External UV detector flow-through cell, low dead volume and low peak width, improve chromatographic resolution.
5. High sensitivity pH, conductivity, and UV detectors to improve chromatographic resolution.
6. Equipped with pressure alarm and bubble sensor detection functions to improve system security and effectively protect chromatography column.
7. The chromatography workstation is provided with computer and software, with powerful functions and advanced performance, which can efficiently and stably manage the chromatographic data. The functions of software comply with the relevant regulations of the CFDA and meet the requirement of the data traceability and security in the pharmaceutical industry.
8. The instrument host and the collector can be stacked to effectively save space, the collector was a closed space to effectively prevent sample contamination.
Technical Parameters
Model | UEV 25D | UEV 25L |
System pump | Material quality | Dual plunger and binary gradient infusion pump, imported PEEK pump head, biological inert material |
Flow rate range | 0.001-25.000 mL/min |
Flow precision | 1 ‰ |
Flow accuracy | ±1 % |
Flow rate reproducibility | ＜0.5 %（RSD） |
Dynamic mixer | 2 mL |
Pressure | 0 - 20 MPa |
Function | Flow rate and ratio can be modified online, supports linear and gradient |
UV detector | Light | Imported deuterium lamp | LED |
Flow-through cell | 2 mm |
Wavelength range | 200-400 nm, simultaneous detection of dual channels | 280nm fixed wavelength detection |
Wavelength accuracy | ±1 nm | ±1 nm |
Wavelength reproducibility | ±0.5 nm | / |
drift | 1×10 -3 AU/Hr | / |
noise | ±4×10 -5 AU（@1s） | ±2×10 -5 AU（@1s） |
pH detector | range：0-14，accuracy：±0.1（@pH=2-12） | No |
Conductivity detector | range：0.001-999.99 mS/cm，accuracy：±1% Compensation for conductivity and temperature |
Temperature Sensor | Built-in conductivity, 0-100 degrees, accuracy: ± 1 degree |
Online filter | Filter buffer for protection |
Mobile phase selection module | Four channels，A1/A2，B1/B2 |
Injection valve | Automatic sampling valve, software control, support Load / Inject / Waste function |
Bubble sensor | Prevent bubbles from entering the system and protect the column |
Two-channel collection valve | 1 collection, 1 waste outlet |
Fraction collector | 1.5 mL and 5 mL 10 × 10 square matrices |
Chromatography workstation | 21.5 inch All-in-one PC, win10 operation system |
Toolkit | Includes PTFE / PEEK tubing, column clamps, operating manuals, tubing connectors, common tools, etc. |
Software functions | Real-time online monitoring of UV, pH, conductivity, temperature and pressure | Real-time online monitoring of UV, conductivity, temperature and pressure |
Real-time modification of flow rate and gradient |
Method editing interface supports UI+Text mode |
Support Chinese and English |
Meet relevant CFDA regulations |
Free software upgrades |
Some customers of UNION protein purifier:
Shanghai light source
East China Normal University
Tianjin Medical University
Beijing University of Chemical Technology
Tsinghua University
Sichuan University
