This product is a screening kit containing 184 conditions based on the phase separation mechanism of proteins and key factors affecting protein phase separation. This product can be used to screen proteins with phase separation ability, evaluate the strength of protein phase separation ability, study the mechanism of protein phase separation and identify the key factors driving protein phase separation. In addition, the effect of other molecules (such as protein, nucleic acid and small molecule) on the phase separation of the target protein can also be identified by pre-mixing the target protein with other molecules.
1.High throughput screening of proteins with phase separation ability.
2.Identify protein phase separation capabilities and phase separation conditions.
3.It can be used to study the molecular mechanism of protein phase separation.
4.Low cost, easy to use, high accuracy.
5.Operators do not need protein phase separation experience or technical background.
1.This product covers the various conditions, including a variety of electrolytes, polymer at the simulation of cell crowded environment, as well as a number of pH gradient, charged reagents.
2.This product can be used to achieve the rapid and efficient screening of phase separation of proteins, while combining a small amount of proteins and reagents added into the phase-separation screening plate, can achieve the quantitative evaluation of the ability to phase separation of the target protein.
3.Low threshold, rapid screening can be achieved by using a blowtorch and an ordinary light-field inverted microscope, and phase separation protein images can be acquired by using a laser confocal microscope.
Phase separation reagent plate
Phase separation screen plate
Note: There are 2 phase separation reagent plates with a total of 23 columns, 8 conditions in each column, and each condition contains 50 μL of solution. Open the sealing cap when using for the first time. After use, it should be quickly sealed with a film and stored at -20 ° C.